Imaging and Analysis of Cells in Flow

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What's New in IDEAS 3.0?

New Features IDEAS Features have undergone extensive changes to provide greater analytical capabilities and ease of use.
 
  • The IDEAS application now contains dozens of new features and improvements on legacy features to strengthen morphological data analysis.
 
  • Users can sort the features by name, category, associated mask, or image using the new Feature Picker.
 
  • Many Features have new, more intuitive names. The IDEAS manual includes detailed documentation on the new, renamed, and deleted features for users upgrading to version 3.0.
New Masks
 
  • Eight new function masks allow the user to define specific areas of interest in a cell and add a greater level of analytical capabilities in IDEAS.
 
  • A new default mask represents more closely what the user would perceive as the object, unlike the previous segmentation mask which attempted to include all visible light. This results in more robust features.
Compensation
 
  • Creating a compensation matrix for .rif control files has never been easier.
    A new Compensation wizard walks you through the process of creating and adjusting a compensation matrix, providing immediate feedback as the matrix is calculated.
  • Choosing the compensation populations is also much easier. IDEAS automatically excludes outliers and saturated events. Compensated imagery is displayed during compensation to assist in validating the matrix.
 
  • You can now adjust the compensation matrix from an open .daf file. You can even recompensate a subset population of a .daf file and view it in a duplicate window of IDEAS.
New Nuclear Translocation Wizard
 
  • Quantifying nuclear translocation has never been easier with the new nuclear translocation wizard that walks you through the analysis.
Reports
 
  • A new statistics report provides users with a powerful tool to process statistical data from multiple files without having to open each file. A statistics report can also be generated during batch processing.

 

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