Temporal Dissection of T-bet Functions
J Matsuda, TC George, J Hagman L Gapin (2007)
Journal of Immunology (2007) 178: 3457.
T-bet is a transcription factor of the T-box family that regulates the expression of numerous immune system-associated genes. In this report the authors produced an ectopically controllable form of T-bet by fusing its C-termial domain with a mutated ligand -binding domain of human estrogen receptor alpha. Using this temporally controllable expression system, they demonstrated that IFN-gamma, CD122 and CxR3 are all direct gene targets of T-bet.
In this study, the ImageStream system was used to demonstrate the subcellular localization of T-bet_ER before and after stimulation with 4-hydroxytamoxifen.
Mitotic Index Multiplexed with Apoptotic Cell Detection
Estimation of the mitotic index, the fraction of the cell population that is in mitosis, is an important and commonly performed assay that usually requires visual examination under a microscope. The low frequency of mitotic cells combined with manual inspection makes the assessment lengthy, tedious and often inaccurate.
The ImageStream system provides a valuable means of measuring the mitotic index of a cell population quickly and accurately. Using high resolution multispectral cell image analysis, the ImageStream can identify and quantitate cell sub-populations based on complex morphological
features. In this application, mitotic cells are identified and distinguished from apoptotic cells.
Because the ImageStream generates cell image data at high speed, a typical analysis can be carried out easily on tens of thousands of cells. These large numbers help
ensure the statistical accuracy of the results.
Amnis Sheath Now Available
New Sheath reagent from Amnis helps insure that your ImageStream system will function optimally. Each lot is fully validated on the ImageStream. Supplied as a case of six 1L bottles.
Catalog No. 600100
MultiLaser Options for the ImageStream system
Now, you can extend the range of fluorescent dyes available to the ImageStream system by adding up to two additional solid state lasers to the ImageStream's standard 488nm laser. The new laser options include a red 658nm laser (90mW) and your choice of either a high power violet 405nm laser (350mW) or a UV 375nm laser (18mW).
Each of these new laser options is available factory installed or they may be purchased for installation on an existing ImageStream system.
To find out more about the benefits of the new ImageStream laser options, please email
lasers@amnis.com
Laser Option
375nm Ultraviolet, 18mW
405nm Violet, 350mW
658nm Red, 90mW
Catalog No.
200110
211120
200130
AAI
American Association for Immunology
Miami Beach Convention Center
Miami, FL
May 18-22, 2007
Booth 409-411
Leukocyte Signal Transduction
Workshop
Aldemar Paradise Village
Rhodes, Greece
June 3-8, 2007
FOCiS
Federation of Clinical Immunology Societies
June 7-9, 2007
San Diego, CA
WNYFUG
Western NY Flow Cytometry Users Group
July 11, 2007
University of Rochester
Rochester, NY
CSBMCB
Canadian Society for Biochemistry, Molecular and Cellular Biology
McGill University
Montreal, Quebec
July 5-9, 2007
Flow Cytometry UK
King's College, Cambridge, UK
July 19-20, 2007
The Stream Line
May 16, 2007
Vol 4 Number 2
The Stream Line is a free, monthly email publication designed to keep you up to date with the applications and technical developments related to the Amnis ImageStream® System. For further information, please visit the Amnis website at www.amnis.com
Copyright © 2007 Amnis Corporation All Rights Reserved
